High Throughput Analysis Laboratory (HTA)

HTA.pngFaculty Director: Eric Weiss, Ph.D.
Managing Director: Chi-Hao Luan, Ph.D.

Location: Silverman Hall B710 & Hogan 4140
Website: sites.northwestern.edu/htal

Overview:

The HTA Laboratory is a shared, open-access resource established in 2004, currently operating in two labs in the Hogan building and a 600 sqft class 100 clean room lab in the Silverman Hall. The HTA provides academic, industrial, and private researchers with equipment and expertise for the development and execution of high throughput biological analysis and screening. The facility is equipped with state-of-the-art liquid handling, plate detection and automated microbial culture handling capabilities.

The HTA routinely performs diverse high throughput applications, including:

  • Automated colony picking and arraying
  • Yeast two-hybrid analysis
  • Synthetic Genetic Array (SGA) analysis
  • Microbial library replication and rearraying
  • Biochemical assays (enzymatic, binding)
  • Cell-based assays (target-directed and phenotypic)
  • Hit picking and library reformatting
  • HTP molecular biology protocols
  • Large scale or complex nature liquid handling
  • Biological and chemical compound library screening
  • HTP fluorescent imaging and quantitative analysis

In addition, the HTA also is a resource for biological and chemical libraries for RNAi and other screening projects, housing two advanced whole-genome shRNAmir libraries (mouse & human) from Open Biosystems, 7 yeast libraries for various knock-out or over-expression experiments, and 60,000 chemical compounds from ChemDiv, ChemBridge, ASDI, and NCI.  The facility also develops novel automated methods to increase assay and sample preparation throughput.